Microbial succession and the dynamics of metabolites and sugars. The final volume of 25 mL of diluted pulp was centrifuged, and 2 mL of, pH 70 ± 02], homogenized for 10 min in a stomacher (Mayo Homogenius HG 400, São, which amplified a fragment of approximately 250 bp (Cocolin, Bisson, & Mills, ), Variations in microbial counts and species depend on the type/variety of.